2010 rfp lactc2 addgene (Addgene inc)

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Addgene inc 2010 rfp lactc2 addgene
2010 Rfp Lactc2 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrfp+lactc2/mRFP-Lact-C2+(Plasmid+%2374061)/10__7554_slash_elife__91345__3-385-11-6
Average 93 stars, based on 18 article reviews

2010 rfp lactc2 addgene - by Bioz Stars, 2026-09

93/100 stars

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Dominant Negative Mutation:

Article Title: The post-abscission midbody is an intracellular signaling organelle that regulates cell proliferation
Article Snippet: .. To generate dominant-negative MFGE-8, MFGE-8-C2 was subcloned from mRFP-LactC2 (Addgene #74061, Sergio Grinstein) and inserted into pGEX-KG. Recombinant GST-tagged protein was eluted with 25 mM glutathione, dialyzed, and used for subsequent assays. .. LifeAct-mCherry was obtained from Addgene (#54491, Michael Davidson).

Recombinant:

Transfection:

Article Title: Role of phosphatidic acid lipids on plasma membrane association of the Ebola virus matrix protein VP40.
Article Snippet: .. HEK293 cells were maintained as previously described [32] and transfected with EGFP-VP40, RFP-PASS, mRFP-LactC2 (from Dr. S. Grinstein, University of Toronto, Addgene #74061), myr-RFP (from Dr. A. Hadjantonakis, Addgene #32604) or mock (plasmid without protein expression insert). ..

Plasmid Preparation:

Expressing:

other:

Article Title: PI4P/PS countertransport by ORP10 at ER-endosome membrane contact sites regulates endosome fission.
Article Snippet: The sources of the other plasmids purchased or kindly provided were: pEBFP-C1 (Takara), pEGFP-C1 (Takara), pEGFP-N1 (Takara), pAcGFP-C1 (Takara), pAcGFP-N1 (Takara), pmCherry-C1 (Takara), pGEX6P1 (Cytiva), p3xFLAG-CMV-10 (Sigma-Aldrich), RFP-Sec61β (Tom Rapoport, Harvard Medical School, Boston, MA), GFP-OSBP-PH (Tim Levine, University College London, London, UK), mCherry2xFyve (Addgene, 140050), GFP-rPI4KIIα (Pietro De Camilli, Yale University, New Haven, CT), iRFP-FRB-Rab5 (Addgene, 51612), iRFP-FRB-Rab7 (Addgene, 51613), GFP-Rab5 (Marino Zerial, Max Planck Institute, Dresden, Germany), GFP-Rab7A (Addgene, 28047), PM-FRB-CFP (Addgene, 67517), mRFP-FKBP12-5ptase domain (Addgene, 67516), mRFP-LactC2 (Addgene, 74061), GFPBioID-FAM21 (Addgene, 121046), pmScarlet-i-C1 (Addgene, 85044), HA-VAPA (Mitsuo Tagaya, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan), pX330S-2-PITCh (Addgene, 63670), pX330A-1x2 (Addgene, 58766), pCRIS-PITChv2-FBL (Addgene, 63672), CI-M6PR (GFP fused to the full-length CI-M6PR [G-CIMPRFull]); Satoshi Waguri, Fukushima Medical University, Fukushima, Japan), GST-LactC2 (Guillaume Drin, Centre national de la recherche scientifique, Valbonne, France), GST–four-phosphate-adaptor protein (FAPP; Guillaume Drin), GST-ORDORP8 (Pietro De Camilli), and Tac-LI (Hiroshi Ohno, RIKEN, Yokohama, Japan).

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